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ACS Chem. Biol., 2008, 3 (1), pp 38–50

General Principles of Synthetic Biology

Reconstruction of genetic circuits

Engineering life: building a fab for biology

Engineering life through Synthetic Biology

Systems biology as a foundation for genome-scale synthetic biology

Synthetic biology: Division of logic labour Cellular compartmentalization is an effective way to build gene circuits capable of complex logic operations, in which binary inputs are converted into binary outputs according to user-defined rules.

Parts Standardization/Construction

Refinement and standardization of synthetic biological parts and devices

Engineering BioBrick vectors from BioBrick parts

Mathematical/Computational Methods

Sensitivity and robustness in chemical reaction networks

New Designs/Design Principles

A synthetic oscillatory network of transcriptional regulators

The incoherent feed-forward loop can generate non-monotonic input functions for genes

In-vivo/vitro Chassis

Protein synthesis by pure translation systems

Principles of cell-free genetic circuit assembly


A synthetic Escherichia coli predator–prey ecosystem

A synthetic multicellular system for programmed pattern formation

Software Packages/Tools

MATLAB SimBiology

Measurement Methods

Single-cell analysis of gene expression by fluorescence microscopy

Ethical, Legal, and Social Issues (ELSI)

Build life to understand it Biologists and engineers should work together: synthetic biology reveals how organisms develop and function, argue Michael Elowitz and Wendell A. Lim.

Diffusion of synthetic biology: a challenge to biosafety. Systems and Synthic Biology

Ethical Considerations in Synthesizing a Minimal Genome